Products

Product Description

Rat Il17a recombinant protein expressed in Escherichia coli.
This product with activity data is belong to bioactive protein.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRSTSPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS

Host

Escherichia coli

Theoretical MW (kDa)

30

Form

Lyophilized

Preparation Method

Escherichia coli expression system

Endotoxin Level

< 0.1 EU/ug

Activity

The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The expected ED50 for this effect is 0.44-0.66 ng/mL.

Quality Control Testing

1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue

Lane 1: non-reducing conditions
Lane 2: reducing conditions

Storage Buffer

Lyophilized from 10 mM sodium citrate, pH 3.0

Storage Instruction

Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis

Result of activity analysis

Serial dilutions of rat Il17a (starting at 2.5 ug/mL) were added to NIH 3T3 cells. After 48 hours, production of mouse IL-6 was measured and the linear portion of the curve was us used to calculate the ED50.