SP1 monoclonal antibody, clone 15H09
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human tonsil tissue stained with SP1 monoclonal antibody, clone 15H09 (Cat # MAB6565) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant SP1.
Immunogen
Recombinant protein corresponding to amino acids 150-200 of human SP1.
Host
Mouse
Theoretical MW (kDa)
90
Reactivity
Human
Specificity
This affinity purified antibody recognizes ~90 KDa of human SP1.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:50)
Flow Cytometry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human tonsil tissue stained with SP1 monoclonal antibody, clone 15H09 (Cat # MAB6565) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SP1
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Interactome
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Pathway
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Disease
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