His Tag monoclonal antibody, clone 6G2A9
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of 6X His Tag.
Antibody Species
Rabbit
Amount
This antibody recognizes His tags localized at the C-terminal, N-terminal, and internal region of fu
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Sequence
HHHHHH
Form
Lyophilized
Purification
Protein A affinity purification
Isotype
IgG1
Recommend Usage
ELISA (0.05-0.2 ug/mL)
Flow Cytometry (1 ug/mL)
Immunocytochemistry (1 ug/mL)
Immunofluorescence (1 ug/mL)
Immunoprecipitation (1 ug/mL)
Western Blot (0.1-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere, lyophilized antibodies are stable up to 1 year at -20°C.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, stored for 2-3 weeks at 2-8°C or for long term storage at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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