SpCas9 Monoclonal Antibody, clone 4A1
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Specification
Product Description
Mouse monoclonal antibody raised against Streptococcus pyogenes Cas9.
Immunogen
Recombinant protein corresponding to Staphylococcus pyogenes Cas9.
Host
Mouse
Reactivity
Streptococcus pyogenes
Specificity
This antibody reacts to spCas9.
Form
Lyophilized
Purification
Protein A affinity purification
Isotype
IgG2a Kappa
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns.
A standard curve should be generated each time the assay is performed.
In this ELISA assay, SpCas9 Monoclonal Antibody was labeled with Biotin. The sensitivity is <1 ng/mL and the detection range is 0-10 ng/mL.Recommend Usage
ELISA (0.01-1 ug/mL)
Immunofluorescence (0.2-1 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (0.02% sodium azide).
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.Storage Instruction
Store at -20°C.
Upon reconstitution, it can be stored for up to 12 months at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Monoclonal Antibody, clone 4A1.
Lane 1: 50 ug cell lysate of HEK293 transfected with PX458
Lane 2: 50 ug untransfected HEK293 cell lysate
Lane 3: 40 ug SpCas9 recombinant protein
Primary Antibody:
SpCas9 Monoclonal Antibody, clone 4A1 1 ug/mL
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 ug/mL -
Gene Info — SPy_1046
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