ATP1B1 monoclonal antibody, clone 464.8
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More Files
- More Functions
Western Blot (Tissue lysate)
Detection of ATP1B1 in rat kidney homogenates (20 ug) using ATP1B1 monoclonal antibody, clone 464.8 (also known as 8A) (Cat # MAB2323).
Lane 1, 1 : 2,500 primary antibody.
Lane 2, 1 : 5,000 primary antibody.
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Specification
Product Description
Mouse monoclonal antibody raised against native ATP1B1.
Immunogen
Native purified rabbit ATP1B1.
Host
Mouse
Reactivity
Bovine, Dog, Human, Pig, Rabbit, Rat
Specificity
This antibody is specific to Na, K-ATPase beta 1 subunit.
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1:2500-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Detection of ATP1B1 in rat kidney homogenates (20 ug) using ATP1B1 monoclonal antibody, clone 464.8 (also known as 8A) (Cat # MAB2323).
Lane 1, 1 : 2,500 primary antibody.
Lane 2, 1 : 5,000 primary antibody. -
Gene Info — ATP1B1
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Publication Reference
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Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.
Winterstein C, Trotter J, Kramer-Albers EM.
Journal of Cell Science 2008 Mar; 121(Pt 6):834.
Application：WB-Ce, Mouse, Mouse oligodendrocytes.
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The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation.
Pessach I, Shmelzer Z, Leto TL, Dinauer MC, Levy R.
Journal of Leukocyte Biology 2006 Sep; 80(3):630.
Application：WB-Ce, Human, X-CGD PLB 91CT cells.
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Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells.
Vagin O, Turdikulova S, Sachs G.
The Journal of Biological Chemistry 2005 Dec; 280(52):43159.
Application：WB, Dog, Human, Pig, HGT-1, LLC-PK1, MDCK cells.
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Use of the H,K-ATPase beta subunit to identify multiple sorting pathways for plasma membrane delivery in polarized cells.
Vagin O, Turdikulova S, Yakubov I, Sachs G.
The Journal of Biological Chemistry 2005 Apr; 280(15):14741.
Application：WB-Tr, Dog, Pig, MDCK, LLC-PK1 cells.
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Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.
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