CD274 monoclonal antibody, clone PDL1/2746
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CD274.
Immunogen
Recombinant protein corresponding to around amino acid 39-191 of human CD274. (exact sequence is proprietary)
Host
Mouse
Theoretical MW (kDa)
37-50
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA (For coating)
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin-fixed) (1-2 ug/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 and (2) HepG2 cell lysates using CD274 monoclonal antibody, clone PDL1/2746.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human Lung SCC using CD274 monoclonal antibody, clone PDL1/2746.Immunofluorescence
Immunofluorescent staining of Jurkat cells labeling CD274 with CD274 monoclonal antibody, clone PDL1/2746.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of human Jurkat cells using CD274 monoclonal antibody, clone PDL1/2746. -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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