Hif1a monoclonal antibody, clone ESEE122 (ATTO 594)
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant mouse Hif1a.
Immunogen
Recombinant protein corresponding to amino acids 329-530 of mouse Hif1a.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Conjugation
ATTO 594
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cells with Hif1a monoclonal antibody, clone ESEE122 (ATTO 594) (Cat # MAB18121).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Hif1a
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