PDE1B monoclonal antibody, clone 5C4A3
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PDE1B.
Immunogen
Recombinant protein corresponding to amino acids 370-536 of human PDE1B from E. coli.
Host
Mouse
Theoretical MW (kDa)
61.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) PDE1B-hIgGFc transfected HEK293 cell lysate with PDE1B monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissue with PDE1B monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PDE1B monoclonal antibody, clone 5C4A3.Flow Cytometry
Flow cytometric analysis of A549 cells with PDE1B monoclonal antibody (green) and negative control (red). -
Gene Info — PDE1B
Entrez GeneID
5153Gene Name
PDE1B
Gene Alias
PDE1B1, PDES1B
Gene Description
phosphodiesterase 1B, calmodulin-dependent
Omim ID
171891Gene Ontology
HyperlinkGene Summary
O
Other Designations
Phosphodiesterase-1B|calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase|calmodulin-stimulated phosphodiesterase PDE1B1|phosphodiesterase IB, calmodulin-dependent|presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family k
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Interactome
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Disease
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