DKK3 monoclonal antibody, clone 8A5C6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DKK3.
Immunogen
Recombinant protein corresponding to amino acids 91-350 of human DKK3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
38.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) DKK3-hIgGFc transfected HEK293 cell lysate with DKK3 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded brain tissue with DKK3 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DKK3 monoclonal antibody, clone 8A5C6. -
Gene Info — DKK3
Entrez GeneID
27122Gene Name
DKK3
Gene Alias
REIC
Gene Description
dickkopf homolog 3 (Xenopus laevis)
Omim ID
605416Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
RIG-like 5-6|RIG-like 7-1|dickkopf 3|dickkopf homolog 3
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