His-Tag chimeric monoclonal antibody, clone RMH01
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of 293T cells (1) transfected a DNA construct encoding His-Tag Histone H3 (G34W) protein, (2) untransfected a DNA construct encoding His-Tag Histone H3 (G34W) protein.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein.
ELISA analysis of a serial dilution of chimeric human anti-HisTag antibody was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of chimeric human His-Tag chimeric monoclonal antibody, clone RMH01 (Cat# MAB16910)1 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
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Specification
Product Description
Human recombinant monoclonal antibody raised against recombinant His-tag.
Antibody Species
Human
Immunogen
Original antibody is raised against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Specificity
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein in mammalian or bacteria cells.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (capturer: 0.1 ug/mL-2 ug/mL)
Detection Antibody (0.01 ug/mL-0.5 ug/mL)
Western Blot (0.1 ug/mL-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot (Transfected lysate) analysis of 293T cells (1) transfected a DNA construct encoding His-Tag Histone H3 (G34W) protein, (2) untransfected a DNA construct encoding His-Tag Histone H3 (G34W) protein.Enzyme-linked Immunoabsorbent Assay
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein.
ELISA analysis of a serial dilution of chimeric human anti-HisTag antibody was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of chimeric human His-Tag chimeric monoclonal antibody, clone RMH01 (Cat# MAB16910)1 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.
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