ENO2 monoclonal antibody, clone ENO2/1375
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Y79, Lane 2: HeLa and Lane 3: HepG2 cell lysates with ENO2 monoclonal antibody, clone ENO2/1375 (Cat # MAB14514).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pheochromocytoma (A), human cerebellum (B), mouse pancreas (C) and rat heart (D) with ENO2 monoclonal antibody, clone ENO2/1375 (Cat # MAB14514).
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human ENO2.
Immunogen
A synthetic peptide corresponding to amino acids 416-433 of human ENO2.
Host
Mouse
Theoretical MW (kDa)
~50
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.2 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Y79, Lane 2: HeLa and Lane 3: HepG2 cell lysates with ENO2 monoclonal antibody, clone ENO2/1375 (Cat # MAB14514).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pheochromocytoma (A), human cerebellum (B), mouse pancreas (C) and rat heart (D) with ENO2 monoclonal antibody, clone ENO2/1375 (Cat # MAB14514).Immunofluorescence
Flow Cytometry
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Gene Info — ENO2
Entrez GeneID
2026Protein Accession#
P09104Gene Name
ENO2
Gene Alias
NSE
Gene Description
enolase 2 (gamma, neuronal)
Omim ID
131360Gene Ontology
HyperlinkGene Summary
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydrolyase|enolase 2|neural enolase|neuron specific gamma enolase|neurone-specific enolase
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Interactomes
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Pathways
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Diseases
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Publication Reference
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DNA sequences encoding enolase are remarkably conserved from yeast to mammals.
Verma M, Dutta SK.
Life Sciences 1994 Jun; 55(12):893.
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DNA sequences encoding enolase are remarkably conserved from yeast to mammals.
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