DUX4 monoclonal antibody, clone P2B1 (ATTO 488)
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant partial DUX4.
Immunogen
Recombinant GST fusion protein corresponding to a 76 amino acid fragment at C-terminus of human DUX4.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes human DUX4. It does not cross-react with DUX4c.
Form
Liquid
Conjugation
ATTO 488
Extinction Coefficient
90,000
Excitation (Max)
501 nm
Emission (Max)
523 nm
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
Application Data with Unconjugated Antibody
Immunofluorescence
Immunofluorescent staining of C2C12 cells transfected pCS2+DUX4 with DUX4 monoclonal antibody, clone P2B1 (Cat # MAB11442) at 10 ug/mL (left).
Counter-stained with DAPI for nuclei (right).Western Blot (Transfected lysate)
Western blot analysis of C2C12 cells transfected with pCS2+DUX4 which contains an additional upstream start site with DUX4 monoclonal antibody, clone P2B1 (Cat # MAB11442).
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Applications
Western Blot (Transfected lysate)
Immunohistochemistry
Immunofluorescence
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Gene Info — DUX4
Entrez GeneID
22947Protein Accession#
NP_149418.3Gene Name
DUX4
Gene Alias
DUX10
Gene Description
double homeobox, 4
Omim ID
606009Gene Ontology
HyperlinkGene Summary
This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length; a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that contains two homeoboxes; the repeat-array and ORF is conserved in other mammals. There was no evidence for transcription from standard cDNA libraries however RTPCR and in-vitro expression experiments indicate that the ORF is transcribed and the encoded protein has been reported to function as a transcriptional activator of paired-like homeodomain transcription factor 1 (PITX1; GeneID 5307). Contraction of the microsatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). [provided by RefSeq
Other Designations
double homeobox protein 4|double homeobox protein DUX10
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Disease
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