HK1 monoclonal antibody, clone 4D7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: HeLa cell lysate; Lane 3: Jurkat cell lysate; Lane 4: K562 cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Hexokinase 1 recombinant protein; Lane 2: Hexokinase 2 recombinant protein; Lane 3: Hexokinase 3 recombinant protein; Lane 4: Hexokinase 4 recombinant protein.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HK1 monoclonal antibody, clone 4D7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HK1.
Immunogen
Recombinant protein corresponding to full length human HK1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: HeLa cell lysate; Lane 3: Jurkat cell lysate; Lane 4: K562 cell lysate.Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Hexokinase 1 recombinant protein; Lane 2: Hexokinase 2 recombinant protein; Lane 3: Hexokinase 3 recombinant protein; Lane 4: Hexokinase 4 recombinant protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HK1 monoclonal antibody, clone 4D7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — HK1
Entrez GeneID
3098GeneBank Accession#
NM_000188Protein Accession#
NP_000179Gene Name
HK1
Gene Alias
HK1-ta, HK1-tb, HK1-tc, HKI, HXK1
Gene Description
hexokinase 1
Omim ID
142600Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
Other Designations
OTTHUMP00000019725|brain form hexokinase|glycolytic enzyme
-
Interactome
-
Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
+ View More Disease
-
Disease
-
Publication Reference
-
Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function.
Wilson JE.
The Journal of Experimental Biology 2003 Jun; 206(Pt 12):2049.
-
Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization.
Furuta H, Nishi S, Le Beau MM, Fernald AA, Yano H, Bell GI.
Genomics 1996 Aug; 36(1):206.
-
Studies on the mechanism of orthophosphate regulation of bovine brain hexokinase.
Ellison WR, Lueck JD, Fromm HJ.
The Journal of Biological Chemistry 1975 Mar; 250(5):1864.
-
Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com