CDKN2A monoclonal antibody, clone 2D9A12
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CDKN2A.
Immunogen
Recombinant protein corresponding to human CDKN2A.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using CDKN2A monoclonal antibody, clone 2D9A12 (Cat # MAB10401) against truncated CDKN2A recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat liver tissue (A), human brain tissue (B) and brain tumor (C), showing nuclear localization using CDKN2A monoclonal antibody, clone 2D9A12 (Cat # MAB10401) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDKN2A
Entrez GeneID
1029Gene Name
CDKN2A
Gene Alias
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19
Gene Description
cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Gene Ontology
HyperlinkGene Summary
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq
Other Designations
CDK4 inhibitor p16-INK4|OTTHUMP00000021147|OTTHUMP00000021148|cell cycle negative regulator beta|cyclin-dependent kinase inhibitor 2A|cyclin-dependent kinase inhibitor p16|multiple tumor suppressor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Ocular Expression and Distribution of Products of the POAG-Associated Chromosome 9p21 Gene Region.
Chidlow G, Wood JP, Sharma S, Dimasi DP, Burdon KP, Casson RJ, Craig JE.
PLoS One 2013 Sep; 8(9):e75067.
Application:IHC-P, WB-Ti, Human, Rat, Brain cortex, Liver, Optic nerve, Retina, Cervical adenocarcinoma.
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Ocular Expression and Distribution of Products of the POAG-Associated Chromosome 9p21 Gene Region.
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