CD14 monoclonal antibody, clone MEM-15 (PE-DyLight™594)
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Specification
Product Description
Mouse monoclonal antibody raised against of CD14.
Immunogen
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Host
Mouse
Theoretical MW (kDa)
53-55
Reactivity
Human, Non-Human Primates
Specificity
CD14, GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.Form
Liquid
Conjugation
PE-DyLight™594
Purification
Size-exclusion chromatography purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 uL reagent/100 uL of whole blood or 106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Surface staining of human peripheral blood cells with CD14 monoclonal antibody, clone MEM-15 (PE) (Cat # MAB5064). -
Gene Info — CD14
Entrez GeneID
929Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Disease
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