NFKBIA monoclonal antibody, clone 6A920
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NFKBIA.
Immunogen
Recombinant protein corresponding to amino acids 32-291 of human NFKBIA.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NFKBIA. Using NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) at 2 ug/mL in (A) Daudi and (B) NIH/3T3 whole cell lysate.Western Blot (Cell lysate)
Jurkat cells were treated for 30 min with 100 ug/mL ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NFKBIA monoclonal antibody, clone 39A1413 (Cat # MAB0056) (lanes 1 & 2) or NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) (that recognizes both non-phospho and phosphorylated forms of IkBa) (lanes 3 & 4) and immunoreactivity was detected by ECL. The data shows that Cat # MAB0056 detects specifically the phosphorylated form of NFKBIA.Immunoprecipitation
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Gene Info — NFKBIA
Entrez GeneID
4792Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
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Publication Reference
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The dNTPase activity of SAMHD1 is important for its suppression of innate immune responses in differentiated monocytic cells.
Qin Z, Bonifati S, St Gelais C, Li TW, Kim SH, Antonucci JM, Mahboubi B, Yount JS, Xiong Y, Kim B, Wu L.
The Journal of Biological Chemistry 2020 Feb; 295(6):1575.
Application:WB-Tr, Human, U-937 cells.
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DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic cells.
Nishioka C, Ikezoe T, Jing Y, Umezawa K, Yokoyama A.
Immunology 2008 Jan; 124(2):198.
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Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia.
Vilimas T, Mascarenhas J, Palomero T, Mandal M, Buonamici S, Meng F, Thompson B, Spaulding C, Macaroun S, Alegre ML, Kee BL, Ferrando A, Miele L, Aifantis I.
Nature Medicine 2006 Dec; 13(1):70.
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BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway.
Endo T, Nishio M, Enzler T, Cottam HB, Fukuda T, James DF, Karin M, Kipps TJ.
Blood 2006 Sep; 109(2):703.
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Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation.
Ishiguro K, Ando T, Maeda O, Hasegawa M, Kadomatsu K, Ohmiya N, Niwa Y, Xavier R, Goto H.
Toxicology and Applied Pharmacology 2006 Jul; 217(1):35.
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NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Santhi WS, Sebastian P, Varghese BT, Prakash O, Pillai MR.
Experimental and Molecular Pathology 2006 Jul; 81(2):123.
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Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome.
E Strozyk, B Poppelmann, T Schwarz, D Kulms.
Oncogene 2006 Oct; 25(47):6239.
Application:WB-Ce, WB-Tr, Human, KB cells.
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p120-catenin mediates inflammatory responses in the skin.
Mirna Perez-Moreno, Michael A Davis, Ellen Wong, H Amalia Pasolli, Albert B Reynolds, Elaine Fuchs.
Cell 2006 Feb; 124(3):631.
Application:WB-Ti, Mouse, Mouse epidermal lysates, Mouse skin.
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The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover.
Lufen Chang, Hideaki Kamata, Giovanni Solinas, Jun-Li Luo, Shin Maeda, K Venuprasad, Yun-Cai Liu, Michael Karin.
Cell 2006 Feb; 124(3):601.
Application:WB-Ce, Mouse, Mouse hepatocytes.
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B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
Min Yang, Hidenori Hase, Diana Legarda-Addison, Leena Varughese, Brian Seed, Adrian T Ting.
Journal of Immunology 2005 Sep; 175(5):2814.
Application:WB-Tr, Mouse, A20 B cells.
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Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1-polarizing program in dendritic cells.
Giorgio Napolitani, Andrea Rinaldi, Francesco Bertoni, Federica Sallusto, Antonio Lanzavecchia.
Nature Immunology 2005 Aug; 6(8):769.
Application:WB-Ce, Human, Human monocyte-derived dendritic cells.
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The IkappaB kinase is a key factor in triggering influenza A virus-induced inflammatory cytokine production in airway epithelial cells.
Daniela Bernasconi, Carla Amici, Simone La Frazia, Angela Ianaro, M Gabriella Santoro.
The Journal of Biological Chemistry 2005 Jun; 280(25):24127.
Application:WB-Tr, Human, A-549 cells.
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Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50.
Yi-Feng Xia, Bu-Qing Ye, Yi-Dan Li, Jian-Guo Wang, Xiang-Jiu He, Xianfeng Lin, Xinsheng Yao, Dawei Ma, Arne Slungaard, Robert P Hebbel, Nigel S Key, Jian-Guo Geng.
Journal of Immunology 2004 Sep; 173(6):4207.
Application:WB-Ce, Human, HEK 293 cells.
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Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response.
Simona Saccani, Ivan Marazzi, Amer A Beg, Gioacchino Natoli.
The Journal of Experimental Medicine 2004 Jul; 200(1):107.
Application:IP, WB-Tr, Human, Mouse, HEK 293T, NIH/3T3 cells.
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PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock.
Takayuki Ikezoe, Yang Yang, David Heber, Hirokuni Taguchi, H Phillip Koeffler.
Molecular Pharmacology 2003 Dec; 64(6):1521.
Application:WB-Ce, Mouse, Raw 264.7 cells.
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NF-kappaB is required for apoptosis prevention during herpes simplex virus type 1 infection.
Margot L Goodkin, Adrian T Ting, John A Blaho.
Journal of Virology 2003 Jul; 77(13):7261.
Application:WB-Tr, Human, HEp-2 cells.
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Modulation of NF-kappaB activity by exchange of dimers.
Simona Saccani, Serafino Pantano, Gioacchino Natoli.
Molecular Cell 2003 Jun; 11(6):1563.
Application:WB-Ce, WB-Tr, Human, HEK 293 cells, Human monocyte-derived dendritic cells.
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p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment.
Saccani S, Pantano S, Natoli G.
Nature Immunology 2002 Jan; 3(1):69.
Application:ChIP, EMSA, WB-Ce, Human, Dendritic cells.
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Two waves of nuclear factor kappaB recruitment to target promoters.
Saccani S, Pantano S, Natoli G.
The Journal of Experimental Medicine 2001 Jun; 193(12):1351.
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The dNTPase activity of SAMHD1 is important for its suppression of innate immune responses in differentiated monocytic cells.
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