ATG10 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ATG10 full-length ORF ( NP_113670.1, 1 a.a. - 220 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLPMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
51.7
Interspecies Antigen Sequence
Mouse (80); Rat (79)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ATG10
Entrez GeneID
83734GeneBank Accession#
NM_031482.3Protein Accession#
NP_113670.1Gene Name
ATG10
Gene Alias
APG10, APG10L, DKFZp586I0418, FLJ13954, pp12616
Gene Description
ATG10 autophagy related 10 homolog (S. cerevisiae)
Omim ID
610800Gene Ontology
HyperlinkGene Summary
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
Other Designations
APG10 autophagy 10-like
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Interactome
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Disease
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