NUF2 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human NUF2 peptide using ARM Technology.
Immunogen
A synthetic peptide of human NUF2 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human NUF2 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — NUF2
Entrez GeneID
83540GeneBank Accession#
NUF2Gene Name
NUF2
Gene Alias
CDCA1, NUF2R
Gene Description
NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
NUF2, NDC80 kinetochore complex component|OTTHUMP00000032177|OTTHUMP00000032178|cell division cycle associated 1
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Interactome
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Disease
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