BOLL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BOLL full-length
Host
Human
Theoretical MW (kDa)
32.56
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BOLL antibody (H00066037-B01) by Western Blots.
SDS-PAGE Gel
BOLL transfected lysate.
Western Blot
Lane 1: BOLL transfected lysate ( 32.56 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BOLL
Entrez GeneID
66037GeneBank Accession#
NM_197970.1Protein Accession#
-Gene Name
BOLL
Gene Alias
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Gene Description
bol, boule-like (Drosophila)
Omim ID
606165Gene Ontology
HyperlinkGene Summary
This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
boule
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Interactome
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Disease
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