STIP1 monoclonal antibody (M01), clone 4B6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant STIP1.
Immunogen
STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (85.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
STIP1 monoclonal antibody (M01), clone 4B6 Western Blot analysis of STIP1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M01), clone 4B6.
Lane 1: STIP1 transfected lysate(62.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3 ug/ml]Immunoprecipitation
Immunoprecipitation of STIP1 transfected lysate using anti-STIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STIP1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STIP1 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — STIP1
Entrez GeneID
10963GeneBank Accession#
BC002987Protein Accession#
AAH02987.1Gene Name
STIP1
Gene Alias
HOP, IEF-SSP-3521, P60, STI1, STI1L
Gene Description
stress-induced-phosphoprotein 1
Omim ID
605063Gene Ontology
HyperlinkGene Summary
STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM
Other Designations
Hsp70/Hsp90-organizing protein|stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Stress-induced phosphoprotein 1 as a secreted biomarker for human ovarian cancer promotes cancer cell proliferation.
Wang TH, Chao A, Tsai CL, Chang CL, Chen SH, Lee YS, Chen JK, Lin YJ, Chang PY, Wang CJ, Chao AS, Chang SD, Chang TC, Lai CH, Wang HS.
Molecular & Cellular Proteomics 2010 Sep; 9(9):1873.
Application:IHC, Human, Ovary.
-
Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis: An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress.
Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T.
Experimental Gerontology 2009 Jun; 44(6-7):375.
Application:WB, Human, SH-SY5Y cells.
-
Novel Breast Cancer Metastasis-Associated Proteins.
Ho J, Kong JW, Choong LY, Loh MC, Toy W, Chong PK, Wong CH, Wong CY, Shah N, Lim YP.
Journal of Proteome Research 2008 Dec; 8(2):583.
Application:WB, Mouse, Mouse 67NR, 168FARN, 4T07, 4T1 cells.
-
Stress-induced phosphoprotein 1 as a secreted biomarker for human ovarian cancer promotes cancer cell proliferation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com