HNMT 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HNMT full-length
Host
Human
Theoretical MW (kDa)
32.23
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HNMT antibody (H00003176-B01) by Western Blots.
SDS-PAGE Gel
HNMT transfected lysate.
Western Blot
Lane 1: HNMT transfected lysate ( 32.23 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HNMT
Entrez GeneID
3176GeneBank Accession#
BC020677.1Protein Accession#
AAH20677.1Gene Name
HNMT
Gene Alias
HMT, HNMT-S1, HNMT-S2
Gene Description
histamine N-methyltransferase
Gene Ontology
HyperlinkGene Summary
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq
Other Designations
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