EPO 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EPO full-length
Host
Human
Theoretical MW (kDa)
21.3
Interspecies Antigen Sequence
Mouse (80); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EPO antibody (H00002056-B01) by Western Blots.
SDS-PAGE Gel
EPO transfected lysate
Western Blot
Lane 1: EPO transfected lysate ( 21.3 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EPO
Entrez GeneID
2056GeneBank Accession#
NM_000799Protein Accession#
NP_000790Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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Pathway
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Disease
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