ALDH1A1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALDH1A1 full-length
Host
Human
Theoretical MW (kDa)
55.22
Interspecies Antigen Sequence
Mouse (87); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALDH1A1 antibody (H00000216-B01) by Western Blots.
SDS-PAGE Gel
ALDH1A1 transfected lysate.
Western Blot
Lane 1: ALDH1A1 transfected lysate ( 55.22 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALDH1A1
Entrez GeneID
216GeneBank Accession#
NM_000689.3Protein Accession#
-Gene Name
ALDH1A1
Gene Alias
ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1
Gene Description
aldehyde dehydrogenase 1 family, member A1
Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq
Other Designations
ALDH class 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase 1A1|aldehyde dehydrogenase, liver cytosolic|retinal dehydrogenase 1|retinaldehyde dehydrogenase 1
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Interactome
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Pathway
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Disease
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