SERPINA3 monoclonal antibody (M01), clone 1E6
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant SERPINA3.
Immunogen
SERPINA3 (AAH03559, 25 a.a. ~ 423 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (58); Rat (62)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SERPINA3 expression in transfected 293T cell line by SERPINA3 monoclonal antibody (M01), clone 1E6.
Lane 1: SERPINA3 transfected lysate(47.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 1 ug/ml]Immunoprecipitation
Immunoprecipitation of SERPINA3 transfected lysate using anti-SERPINA3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINA3 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SERPINA3 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — SERPINA3
Entrez GeneID
12GeneBank Accession#
BC003559Protein Accession#
AAH03559Gene Name
SERPINA3
Gene Alias
AACT, ACT, GIG24, GIG25, MGC88254
Gene Description
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Omim ID
107280Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq
Other Designations
alpha-1-antichymotrypsin|antichymotrypsin|growth-inhibiting protein 24|growth-inhibiting protein 25|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3|serine (or cysteine) proteinase inhibitor, clade A, memb
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Interactomes
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Diseases
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