COVID-19 Beta Variant Pseudovirus Neutralizing Antibody Assay (Luciferase)
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More Files
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Neutralization
RBD-binding IgG responses in mouse sera collected 35 days and 42 days after immunization with 5 ug or 20 ug COVID-19 RBD SAM LNP no adjuvant (ppopV0) and 5 ug or 20 ug COVID-19 RBD SAM plus adjuvant (ppopV1).
Neutralization
SARS-CoV-2 beta variant spike pseudovirus expressing luciferase is treated with humanized monoclonal antibody 7F7 and LALAPG 7F7, and then added into 293T-ACE cells.
In vivo Neutralization
Hamsters received 2 doses of humanized monoclonal antibody 7F7 and then were infected with SARS-CoV-2 Bata Variant Pseudovirus Expressing Luciferase (Cat # U0403).
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Specifications
Product Description
COVID-19 Beta Variant Pseudovirus Neutralizing Antibody Assay (Luciferase) provides a highly sensitive and quantitative measurement of neutralizing antibodies.
Supplied Product
Kit content:
1 kit is sufficient for 100 assays in 24 well plates
1. 293T-hACE2 cell, 2x106cells/vial, FBS with 10% DMSO
2. SARS-CoV-2 Beta Variant Pseudovirus Expressing LuciferaseMaterials Required But NOT Supplied
1. SARS-CoV-2 Monoclonal Antibody, Cat# MAB22854-M07, Abnova
2. ZymeFree™ Enzyme Free Cell Dissociation Reagent,
Cat# TCL028-100ML, HIMEDIA
3. DMEM culture medium containing 10% FBS (complete medium)
4. Luciferase Assay System, Cat# E1500, Promega
a. Luciferase Assay Substrate (lyophilized)
b. Luciferase Assay Buffer
c. Luciferase Cell Culture Lysis Reagent, 5X
5. TC-Treated 24 Well Plates, Cat# 3524, Corning
6. OptiPlate™-96, Cat# 6005290, PerkinElmerVariant
B.1.351
Suitable Sample
1. Immunized Serum
2. Infected Serum
3. Neutralizing Antibodies
4. Peptide Inhibitors or Proteins
5. Compounds Targeting Spike Induced Cell FusionSample Volume
10-50 uL (please check sample source)
Detection Method
Luciferase Activity
Technology
Regulatory Status
For research use only (RUO)
Storage Instruction
293T-hACE2 cell Store at liquid nitrogen.
Pseudovirus Store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Neutralization
RBD-binding IgG responses in mouse sera collected 35 days and 42 days after immunization with 5 ug or 20 ug COVID-19 RBD SAM LNP no adjuvant (ppopV0) and 5 ug or 20 ug COVID-19 RBD SAM plus adjuvant (ppopV1).Neutralization
SARS-CoV-2 beta variant spike pseudovirus expressing luciferase is treated with humanized monoclonal antibody 7F7 and LALAPG 7F7, and then added into 293T-ACE cells.In vivo Neutralization
Hamsters received 2 doses of humanized monoclonal antibody 7F7 and then were infected with SARS-CoV-2 Bata Variant Pseudovirus Expressing Luciferase (Cat # U0403).
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