Products

Product Description

ERBB2 CISH Probe is designed for the qualitative detection of human ERBB2 gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Reactivity

Human

Recommend Usage

The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.

Supplied Product

Reagent Provided:

1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 17q12* (chr17:37,725,661-37,973,541) harboring the ERBB2 gene
2. Formamide based hybridization buffer

*according to Human Genome Assembly GRCh37/hg19

Probe Position

Regulatory Status

For research use only (RUO)

Storage Instruction

Store at 2-8°C in an upright position. Return to storage conditions immediately after use.

Note

The probe is intended to be used in combination with the CISH Implementation Kit 1 (Catalog #: KA5365), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (ERBB2 gene region).
Normal situation: In interphases of normal cells or cells without an amplification involving the ERBB2 gene locus, two distinct dot-shaped brown signals per nuclei appear.
Aberrant situation: In a cell with amplification of the ERBB2 gene locus or polysomy of chromosome 17, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Interpretation of Result

Self-Attestation

Abnova self-attests to comply with the U.S. Framework for Nucleic Acid Synthesis Screening