AO solution
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Immunofluorescence
HeLa Cell staining with AO
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Specification
Product Description
Acridine orange (AO) solution is used as nuclear dye. AO forms a complex with double-stranded DNA to emit green fluorescence, AO also forms a complex with singlestranded DNA or RNA to emit red fluorescence. One molecule of AO intercalates with three base pairs of double-stranded DNA and emits green fluorescence with the maximum wavelength at 526 nm (excitation 502 nm). One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked, structure that emits red fluorescence with the maximum wavelength at 650 nm (excitation 460 nm).
Theoretical MW (kDa)
301.81
Form
Liquid
Regulation Status
For research use only (RUO)
Storage Instruction
Store at -20°C. Protect from light.
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Applications
Immunofluorescence
HeLa Cell staining with AOFlow Cytometry
Fluorescence Microscopy Experiment
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