RNase Inhibitor
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Specification
Product Description
Murine RNase inhibitor binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the enzymes and protecting RNA from degradation.
Host
Escherichia coli
Preparation Method
Escherichia coli expression system
Endotoxin Level
≤ 10 EU/mg
Activity
The amount of murine RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50% was defined as one unit (U)
Quality Control Testing
Exonuclease Activity: 40 U of murine RNaseinhibitor with 1 ug Lambda-Hind III digest DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Endonuclease Activity: 40 U of murine RNase inhibitor with 1 ug Lambda DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Nicking Activity: 40 U of murine RNase inhibitor with 1 ug pBR322 at 37°C for 16 hours yields no degradation as determined by agarose gel elStorage Buffer
In 50 mM KCl, 20 mM HEPES-KOH, pH 7.6 (8 mM DTT and 50% glycerol)
Storage Instruction
Store at -20°C.
Note
It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus.
Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That making it stable at low concentrations of DTT (less than 1 mM).
The optimum temperature range of this the inhibitor was 25-55°C, and It was inactivated at 65°C and above.
The activities of RNase H, RNase 1 and RNase T1 were not inhibited by murine RNase_x005F_x005F_x005F_x000D__x005F_x000D_ inhibitor.
The inhibition of RNase activity was found in a wide range of pH (pH 5-9 were all active), and the highest activity was observed at pH 7-8. -
Applications
Functional Study
In vitro Transcription
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