TUBB1 recombinant monoclonal antibody, clone 20B1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TUBB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human TUBB1.
Theoretical MW (kDa)
Calculated MW: 50
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of TUBB1 recombinant monoclonal antibody, clone 20B1 diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing NIH/3T3 cells stained with EGFR recombinant monoclonal antibody, clone 29C10 (red line) at 1:200. -
Gene Info — TUBB1
Entrez GeneID
81027Protein Accession#
Q9H4B7Gene Name
TUBB1
Gene Alias
dJ543J19.4
Gene Description
tubulin, beta 1
Gene Ontology
HyperlinkGene Summary
Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM
Other Designations
OTTHUMP00000031411|beta tubulin 1, class VI
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