ACAA2 recombinant monoclonal antibody, clone 12F4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: A549 whole cell lysate; Lane3: Mouse thymus.
Immunohistochemistry
Immunohistochemistry image of ACAA2 recombinant monoclonal antibody, clone 12F4 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with ACAA2 recombinant monoclonal antibody, clone 12F4 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse ACAA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ACAA2.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: A549 whole cell lysate; Lane3: Mouse thymus.Immunohistochemistry
Immunohistochemistry image of ACAA2 recombinant monoclonal antibody, clone 12F4 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with ACAA2 recombinant monoclonal antibody, clone 12F4 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACAA2
Entrez GeneID
10449Protein Accession#
P42765Gene Name
ACAA2
Gene Alias
DSAEC, FLJ35992, FLJ95265
Gene Description
acetyl-Coenzyme A acyltransferase 2
Omim ID
604770Gene Ontology
HyperlinkGene Summary
The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq
Other Designations
acetyl-coenzyme A acyltransferase 2|beta ketothiolase|mitochondrial 3-oxoacyl-CoA thiolase|mitochondrial 3-oxoacyl-Coenzyme A thiolase
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Interactome
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Pathway
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Disease
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