RPS19 recombinant monoclonal antibody, clone 7E6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HEK293 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, A549 whole cell lysate with RPS19 recombinant monoclonal antibody, clone 7E6 (Cat # RAB07579).
Flow Cytometry
Flow cytometry shows HepG2 cells stained with RPS19 recombinant monoclonal antibody, clone 7E6 (Cat # RAB07579)(red line). The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RPS19.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human RPS19.
Theoretical MW (kDa)
Calculated MW: 17
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HEK293 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, A549 whole cell lysate with RPS19 recombinant monoclonal antibody, clone 7E6 (Cat # RAB07579).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry shows HepG2 cells stained with RPS19 recombinant monoclonal antibody, clone 7E6 (Cat # RAB07579)(red line). The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. -
Gene Info — RPS19
Entrez GeneID
6223Protein Accession#
P39019Gene Name
RPS19
Gene Alias
DBA
Gene Description
ribosomal protein S19
Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S19|Diamond-Blackfan anemia
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Interactome
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Pathway
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Disease
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