CCR4 recombinant monoclonal antibody, clone 12F5
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More Files
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Flow Cytometry
Flow cytometry analysis of untransfected HEK293 cells (green line) and transfected human CCR4 HEK293 stable cells (red line) stained with CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated anti-Human IgG Fc antibody.
The ELISA results show that the binding activity of CCR4 with CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552). Immobilized CCR4 (CSB-MP004843HU) at 10 ug/mL can bind CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552), the EC50 is 362.3-630.8 ng/mL.
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Specification
Product Description
Human recombinant monoclonal antibody raised against human CCR4.
Antibody Species
Human
Immunogen
Original antibody is raised against recombinant protein of human CCR4.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG1
Quality Control Testing
Binding activity
The ELISA results show that the binding activity of CCR4 with CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552). Immobilized CCR4 (CSB-MP004843HU) at 10 ug/mL can bind CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552), the EC50 is 362.3-630.8 ng/mL.
Recommend Usage
ELISA
Flow Cytometry(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (0.03% Proclin 300 and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of untransfected HEK293 cells (green line) and transfected human CCR4 HEK293 stable cells (red line) stained with CCR4 recombinant monoclonal antibody, clone 12F5 (Cat # RAB07552)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated anti-Human IgG Fc antibody. -
Gene Info — CCR4
Entrez GeneID
1233Protein Accession#
P51679Gene Name
CCR4
Gene Alias
CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5, MGC88293
Gene Description
chemokine (C-C motif) receptor 4
Omim ID
604836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq
Other Designations
chemokine (C-C) receptor 4
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Interactome
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Pathway
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Disease
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