CLDN18 recombinant monoclonal antibody, clone 9C2
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More Files
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Flow Cytometry
Flow cytometry analysis of untransfected KATO3 cells (green line), transfected human CLDN18.2 KATO3 stable cells (red line) and transfected human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated Goat Anti-Human IgG, Fc-gamma antibody (CSB-PA271513).
The ELISA results show that the binding activity of human CLDN18.2 with CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551). Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 ug/mL can bind CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551), the EC50 is 6.554-27.87 ng/mL.
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Specification
Product Description
Human recombinant monoclonal antibody raised against human CLDN18.
Antibody Species
Human
Immunogen
Original antibody is raised against recombinant protein of human CLDN18.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG1
Quality Control Testing
Binding activity
The ELISA results show that the binding activity of human CLDN18.2 with CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551). Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 ug/mL can bind CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551), the EC50 is 6.554-27.87 ng/mL.
Recommend Usage
ELISA
Flow Cytometry(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (0.03% Proclin 300 and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of untransfected KATO3 cells (green line), transfected human CLDN18.2 KATO3 stable cells (red line) and transfected human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with CLDN18 recombinant monoclonal antibody, clone 9C2 (Cat # RAB07551)(2ug/1*106cells). The cells were washed and then followed by FITC-conjugated Goat Anti-Human IgG, Fc-gamma antibody (CSB-PA271513). -
Gene Info — CLDN18
Entrez GeneID
51208Protein Accession#
P56856-2Gene Name
CLDN18
Gene Alias
SFTA5, SFTPJ
Gene Description
claudin 18
Omim ID
609210Gene Ontology
HyperlinkGene Summary
CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001 [PubMed 11585919]).[supplied by OMIM
Other Designations
surfactant associated 5|surfactant associated protein J|surfactant, pulmonary associated protein J
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Pathway
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