MBD1 recombinant monoclonal antibody, clone R02-7E9
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MBD1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against protein corresponding to full length human MBD1.
Theoretical MW (kDa)
Calculated MW: 67 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end use.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — MBD1
Entrez GeneID
4152Protein Accession#
Q9UIS9Gene Name
MBD1
Gene Alias
CXXC3, PCM1, RFT
Gene Description
methyl-CpG binding domain protein 1
Omim ID
156535Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. [provided by RefSeq
Other Designations
OTTHUMP00000163504|OTTHUMP00000163506|OTTHUMP00000163507|methyl-CpG binding domain protein 1 isoform PCM1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
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Interactome
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Disease
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