CHAF1A recombinant monoclonal antibody, R06-3A5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CHAF1A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CHAF1A.
Theoretical MW (kDa)
Calculated MW: 107 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunohistochemistry (Frozen sections) (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 lysates with CHAF1A recombinant monoclonal antibody, R06-3A5 (Cat # RAB04907).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CHAF1A recombinant monoclonal antibody, R06-3A5 (Cat # RAB04907). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — CHAF1A
Entrez GeneID
10036Protein Accession#
Q13111Gene Name
CHAF1A
Gene Alias
CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
Gene Description
chromatin assembly factor 1, subunit A (p150)
Omim ID
601246Gene Ontology
HyperlinkGene Summary
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM
Other Designations
chromatin assembly factor I (150 kDa)
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Interactome
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Disease
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