HNRNPC recombinant monoclonal antibody, clone 9G1
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: MCF-7 whole cell lysate, Lane 5: Raji whole cell lysate, Lane 6: Jurkat whole cell lysate and Lane 7: HepG2 whole cell lysate with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362).
Immunohistochemistry
Immunohistochemical staining of human kidney tissue with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:66.35).
Immunofluorescence
Immunofluorescent staining of Hela cells with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:22). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362).
Lane 1: rabbit control IgG, Lane 2: RAB04362 precipitates and Lane 3: Input (Hela whole cell lysate).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HNRNPC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HNRNPC.
Theoretical MW (kDa)
Calculated MW: 34, 3
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: MCF-7 whole cell lysate, Lane 5: Raji whole cell lysate, Lane 6: Jurkat whole cell lysate and Lane 7: HepG2 whole cell lysate with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362).Immunohistochemistry
Immunohistochemical staining of human kidney tissue with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:66.35).Immunofluorescence
Immunofluorescent staining of Hela cells with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:22). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362).
Lane 1: rabbit control IgG, Lane 2: RAB04362 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with HNRNPC recombinant monoclonal antibody, clone 9G1 (Cat # RAB04362) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — HNRNPC
Entrez GeneID
3183Protein Accession#
P07910Gene Name
HNRNPC
Gene Alias
C1, C2, HNRNP, HNRPC, MGC104306, MGC105117, MGC117353, MGC131677, SNRPC
Gene Description
heterogeneous nuclear ribonucleoprotein C (C1/C2)
Omim ID
164020Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq
Other Designations
heterogeneous nuclear ribonucleoprotein C|nuclear ribonucleoprotein particle C1 protein|nuclear ribonucleoprotein particle C2 protein
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