VEGFA recombinant monoclonal antibody, clone VG76e
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human VEGF189 expressed in E. coli.
Antibody Species
Mouse
Immunogen
Original antibody is raised against recombinant protein corresponding to human VEGF189 expressed in E. coli.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of rat thyroid, rat bladder, mouse brain, human thyroid cancer, human ovarian cancer lysate using anti-VEGF recombinant monoclonal antibody, clone VG76e (Cat # RAB03962).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
P15692Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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