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Western blot analysis of Lane 1: mouse spleen, Lane 2: mouse thymus and Lane 3: mouse lung with Tnfrsf14 recombinant monoclonal antibody, clone HMHV-1B18 (Cat # RAB03548).
Western Blot analysis the tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots probed with the chimeric r version of RAB03548 at 0.01 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of RAW264.7 cells with Tnfrsf14 recombinant monoclonal antibody, clone HMHV-1B18 (Cat # RAB03548).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03548 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03548
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of mouse splenocytes with Tnfrsf14 recombinant monoclonal antibody, clone HMHV-1B18 (Cat # RAB03548).
Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03548 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.