Cd8a recombinant monoclonal antibody, clone YTS 169.4
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More Files
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Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Cd8a recombinant monoclonal antibody, clone YTS 169.4 (Cat # RAB03352).
Mouse lymphocytes were stained with an isotype control (panel A) or the r version of RAB03352 (panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing the bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analyzed using a FACSCanto flow-cytometer.
Flow Cytometry
Flow cytometric analysis of Mouse thymocytes with Cd8a recombinant monoclonal antibody, clone YTS 169.4 (Cat # RAB03352).
Mouse thymocytes were stained with unimmunized r antibody (isotype control-black line) or the r version of RAB03352 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd8a.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against murine CD8 (lyt-2).
Reactivity
Mouse
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG, kappa
Recommend Usage
Depletion
Flow Cytometry
Immunohistochemistry
Immunohistochemistry (Frozen sections)
In vivo Imaging
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Frozen sections)
Immunohistochemistry
Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Cd8a recombinant monoclonal antibody, clone YTS 169.4 (Cat # RAB03352).
Mouse lymphocytes were stained with an isotype control (panel A) or the r version of RAB03352 (panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing the bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analyzed using a FACSCanto flow-cytometer.Flow Cytometry
Flow cytometric analysis of Mouse thymocytes with Cd8a recombinant monoclonal antibody, clone YTS 169.4 (Cat # RAB03352).
Mouse thymocytes were stained with unimmunized r antibody (isotype control-black line) or the r version of RAB03352 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.In vivo Imaging
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Gene Info — Cd8a
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