Cd74 recombinant monoclonal antibody, clone In-1
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More Files
- More Functions
Western Blot
Western blot anaysis of Lane 1: mouse spleen lysate and Lane 2: mouse lymph node lysate with Cd74 recombinant monoclonal antibody, clone In-1 (Cat # RAB03327).
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd74 recombinant monoclonal antibody, clone In-1 (Cat # RAB03327).
Mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version ofRAB03327 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd74.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse lymphocytes of strain A injected into Wistar rats.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot anaysis of Lane 1: mouse spleen lysate and Lane 2: mouse lymph node lysate with Cd74 recombinant monoclonal antibody, clone In-1 (Cat # RAB03327).Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd74 recombinant monoclonal antibody, clone In-1 (Cat # RAB03327).
Mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version ofRAB03327 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — Cd74
Entrez GeneID
16149Gene Name
Cd74
Gene Alias
CLIP, DHLAG, HLADG, Ia-GAMMA, Ii
Gene Description
CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
CD74 antigen|HLA-DR-GAMMA|Ia-associated invariant chain|MHC class II-associated invariant chain|class II-associated invariant chain peptide|dinucleotide microsatellite|histocompatibility: class II antigens, gamma chain of|invariant polypeptide of major hi
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