Ccrl2 recombinant monoclonal antibody, clone BZ5B8
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: mouse thymus and Lane 2: mouse brain tissue lysates with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03142 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03142- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).
RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03142 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Ccrl2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mCCRL2 peptide/KLH conjugate.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Functional Study
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: mouse thymus and Lane 2: mouse brain tissue lysates with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03142 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03142- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Functional Study
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Ccrl2 recombinant monoclonal antibody, clone BZ5B8 (Cat # RAB03142).
RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03142 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Ccrl2
Entrez GeneID
54199Gene Name
Ccrl2
Gene Alias
1810047I05Rik, CCR11, Cmkbr1l2, E01, L-CCR
Gene Description
chemokine (C-C motif) receptor-like 2
Gene Ontology
HyperlinkGene Summary
-like 2|chemokine (C-C) receptor 1-like 2|lipopolysaccharide inducible C-C chemokine receptor related
Other Designations
chemokine (C-C) receptor 1,-like 2|chemokine (C-C) receptor 1-like 2|lipopolysaccharide inducible C-C chemokine receptor related
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