Birch pollen allergen recombinant monoclonal antibody, clone M0418
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone M0418 (Cat # RAB03102).
Binding curve of RAB03102 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03102. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03102 has differing affinities.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against Birch pollen allergen (BETVIA).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against Birch pollen allergen, a major allergen of the European white birch.
Reactivity
Plants
Form
Liquid
Isotype
IgG lambda
Recommend Usage
Crystallography
ELISA
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone M0418 (Cat # RAB03102).
Binding curve of RAB03102 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03102. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03102 has differing affinities.Crystallography
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