H3C1 recombinant monoclonal antibody, clone R01-8I4
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More Files
- More Functions
Western Blot
Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.
Immunocytochemistry
Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody,and DAPI(blue)
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human H3C1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3.
Theoretical MW (kDa)
Calculated MW: 15 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
ChIP
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot
Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody,and DAPI(blue)Immunofluorescence
Immunoprecipitation
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Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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