SYN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k SYN1 protein. Using SYN1 polyclonal antibody (Cat # PAB9702).
Immunofluorescence
Immunochemical staining of cultured caudate neurons with SYN1 polyclonal antibody (Cat # PAB9702) (green) and anti-MAP antibody (red). Cells and phospho courtesy of QBM Cell Science.
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Specification
Product Description
Rabbit polyclonal antibody raised against native SYN1.
Immunogen
Native purified bovine SYN1.
Host
Rabbit
Theoretical MW (kDa)
78
Reactivity
Bovine, Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:2000)
Immunofluorescence (1:2000)
Immunoprecipitation (1 ug/200 ug lysate )
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 5 mM ammonium bicarbonate
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with PBS, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k SYN1 protein. Using SYN1 polyclonal antibody (Cat # PAB9702).Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunochemical staining of cultured caudate neurons with SYN1 polyclonal antibody (Cat # PAB9702) (green) and anti-MAP antibody (red). Cells and phospho courtesy of QBM Cell Science.Immunoprecipitation
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Gene Info — SYN1
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Publication Reference
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Regulation of neurotransmitter release by synapsin III.
Feng J, Chi P, Blanpied TA, Xu Y, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P.
Journal of Neuroscience 2002 Jun; 22(11):4372.
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A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P.
Nature Neuroscience 2002 Apr; 5(5):431.
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Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals.
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC Jr, Greengard P, Czernik AJ.
The Journal of Neuroscience 2001 Oct; 21(20):7944.
Application：WB, Rat, Synaptosomes generated from cerebral cortices.
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Regulation of neurotransmitter release by synapsin III.
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