IFT88 polyclonal antibody
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More Files
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of IFT88.
Immunogen
A synthetic peptide corresponding to C-terminus of human IFT88.
Sequence
C-KKRIDEDDFADEE
Host
Goat
Theoretical MW (kDa)
94.3, 93.2
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_783195.2, NP_006522.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IFT88 polyclonal antibody (Cat # PAB6947) (3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — IFT88
Entrez GeneID
8100Protein Accession#
NP_783195.2;NP_006522.2Gene Name
IFT88
Gene Alias
D13S1056E, DAF19, MGC26259, RP11-172H24.2, TG737, TTC10, hTg737
Gene Description
intraflagellar transport 88 homolog (Chlamydomonas)
Omim ID
600595Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
TPR repeat protein 10|intraflagellar transport 88 homolog|polaris|probe hTg737 (polycystic kidney disease, autosomal recessive)|recessive polycystic kidney disease protein Tg737 homolog|tetratricopeptide repeat domain 10
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Interactomes
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Diseases
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Publication Reference
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Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.
Jiang ST, Chiou YY, Wang E, Chien YL, Ho HH, Tsai FJ, Lin CY, Tsai SP, Li H.
Human Molecular gGnetics 2009 May; 18(9):1566.
Application:IF, Mouse, Retinal.
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News focus: Betting on cilia.
Gretchen Vogel.
Science 2005 Oct; 310(5746):216.
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Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.
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