SKAP2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SKAP2.
Immunogen
A synthetic peptide corresponding to human SKAP2.
Sequence
C-GLVPKAYIMEMYDI
Host
Goat
Theoretical MW (kDa)
41.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SKAP2 polyclonal antibody (Cat # PAB6127) staining (0.5 ug/mL) of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SKAP2 (RC206119) and probed with EB05356 (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SKAP2
Entrez GeneID
8935Protein Accession#
NP_003921.2Gene Name
SKAP2
Gene Alias
MGC10411, MGC33304, PRAP, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R
Gene Description
src kinase associated phosphoprotein 2
Omim ID
605215Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the src family kinases. This protein is similar to the src kinase associated phosphoprotein 1. It is an adaptor protein that is thought to play an essential role in the src signaling pathway in various cells. It inhibits PTK2B/RAFTK activity and regulates alpha-synuclein phosphorylation. [provided by RefSeq
Other Designations
Fyn-associated phosphoprotein SKAP55 homologue|Pyk2/RAFTK-associated protein|src family associated phosphoprotein 2|src kinase-associated phosphoprotein of 55-related protein|src-associated adaptor protein
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Interactome
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Disease
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Publication Reference
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Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation.
Takahashi T, Yamashita H, Nagano Y, Nakamura T, Ohmori H, Avraham H, Avraham S, Yasuda M, Matsumoto M.
The Journal of Biological Chemistry 2003 Jul; 278(43):42225.
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Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation.
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