TAOK3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TAOK3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TAOK3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of TAOK3 (arrow) using rabbit TAOK3 polyclonal antibody (Cat # PAB4527). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with TAOK3 polyclonal antibody (Cat # PAB4527) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — TAOK3
Entrez GeneID
51347Protein Accession#
NP_057365;Q9H2K8Gene Name
TAOK3
Gene Alias
DKFZp666H245, DPK, FLJ31808, JIK, MAP3K18
Gene Description
TAO kinase 3
Gene Ontology
HyperlinkOther Designations
STE20-like kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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SEREX identification of new tumour-associated antigens in cutaneous T-cell lymphoma.
Hartmann TB, Thiel D, Dummer R, Schadendorf D, Eichmuller S.
The British Journal of Dermatology 2004 Feb; 150(2):252.
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Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38.
Yustein JT, Xia L, Kahlenburg JM, Robinson D, Templeton D, Kung HJ.
Oncogene 2003 Sep; 22(40):6129.
Application:KA, WB-Tr, Human, COS-1 cells.
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Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress.
Yoneda T, Imaizumi K, Oono K, Yui D, Gomi F, Katayama T, Tohyama M.
The Journal of Biological Chemistry 2001 Apr; 276(17):13935.
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SEREX identification of new tumour-associated antigens in cutaneous T-cell lymphoma.
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