Cas9 polyclonal antibody
* The price is valid only in USA. Please select country.
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Cas9 polyclonal antibody of 293T cells at 1:1000 dilution. Secondary antibody: HRP Goat Ant-Rabbit IgG (H+L) at 1:10000 dilution. Lysate/proteins: 25 ug per lane.
Immunofluorescence
Immunoflourescence analysis of Cas9 polyclonal antibody of Hela cells transfected with Cas9 (upper left) and untreated Hela cells (upper right) using Cas9 polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant cas9 protein.
Host
Rabbit
Reactivity
Streptococcus pyogenes
Form
Liquid
Purification
Affinity purification
Recommend Usage
CRISPR Genome editing
Immunofluorescence (1:50 - 1:200)
Western Blot (1:5000 - 1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% Glycerol, pH 7.3).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Cas9 polyclonal antibody of 293T cells at 1:1000 dilution. Secondary antibody: HRP Goat Ant-Rabbit IgG (H+L) at 1:10000 dilution. Lysate/proteins: 25 ug per lane.Immunofluorescence
Immunoflourescence analysis of Cas9 polyclonal antibody of Hela cells transfected with Cas9 (upper left) and untreated Hela cells (upper right) using Cas9 polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.CRISPR Genomic Editing
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