SARS-CoV-2 M polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against partial synthetic peptide of SARS-CoV-2 M.
Immunogen
A synthetic peptide corresponding to amino acids 161-210 of SARS-CoV-2 M.
Host
Rabbit
Reactivity
SARS-CoV-2
Specificity
The antibody detects endogenous levels of SARS-CoV-2 M.
Form
Liquid
Purification
Affinity purification
Purity
> 95% by SDS-PAGE
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of SARS-CoV-2 M polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate (negative control), Lane 2: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His. Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell lysate, Lane 4: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains.
Dylan Juckel, Lowiese Desmarets, Adeline Danneels, Yves Rouillé, Jean Dubuisson, Sandrine Belouzard.
The Journal of General Virology 2023 Oct; 104(10).
Application:IF, WB, Human, Huh-7 cells.
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F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells.
Jitendriya Swain, Peggy Merida, Karla Rubio, David Bracquemond, Aymeric Neyret, Israel Aguilar-Ordoñez, Stefan Günther, Guillermo Barreto, Delphine Muriaux.
iScience 2023 Jul; 26(8):107384.
Application:IF, Human, A549-hACE2 cells.
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MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains.
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