Chicken anti-Goat IgG secondary antibody (HRP)
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Specification
Product Description
Chicken polyclonal antibody raised against Goat IgG. The antibody is conjugated with HRP.
Immunogen
Purified goat IgG (whole protein) emulsified in Freund's adjuvant.
Host
Chicken
Reactivity
Goat
Form
Liquid
Conjugation
HRP
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
This antibody was tested for specificity using double- immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.
Recommend Usage
ELISA
Western Blot(1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM HEPES, pH 7.0, 0.1M NaCl, (0.2% BSA, 0.01% thimerosal).
Storage Instruction
Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosol: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
Anil Kumar Vijjamarri, Neha Gupta, Chisom Onu, Xiao Niu, Fan Zhang, Rakesh Kumar, Zhenguo Lin, Miriam L Greenberg, Alan G Hinnebusch.
Nucleic Acids Research 2023 Jul; [Epub].
Application：WB, Yeast, dhh1 Δstrain H5217, pat1 Δstrain F2181, WT strain F2147.
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mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
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